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Gut Microbiota Effects On Bone Density Are Dependent On T‐ and B‐ lymphocytes
Author(s) -
RiosArce Naiomy Deliz,
Schepper Jonathan,
McCabe Laura R.,
Parameswaran Narayanan
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.924.3
Subject(s) - gut flora , abx test , medicine , biology , immune system , endocrinology , immunology , statistics , mathematics
Recent studies have shown that gut microbiota is an important regulator of bone density. In addition, it is well known that the gut microbiota can modulate the immune system. However, whether the effects of the gut microbiota on bone health involve the lymphocytes is still unknown. We hypothesized that modulation of gut microbiota will alter bone density, and this effect will be prevented in the absence of lymphocytes. To test our hypothesis, we treated Rag knockout (T and B lymphocyte deficient) mice and the corresponding C57BL/6J wild‐type mice (males, 12 weeks of age) with antibiotics (ABX) ampicillin (1g/L) and neomycin (0.5g/L) in the water for two weeks. After two weeks, mice received water without antibiotics for another 4 weeks to allow the repopulation of gut microbiota. Bone volume fractions (BVF) of the femur were analyzed using microcomputed tomography system. Fecal samples were used to confirm microbiota depletion after 2 weeks and repopulation after 6 weeks. Repopulation of the gut microbiota in the WT‐ABX group significantly decreased femoral trabecular BVF by 24% compared to the non‐ABX treated WT group (BVF/BW WT:.98, WT‐ABX:.74, n= 14–17). In the Rag KO mice, however, repopulation of the gut microbiota following ABX treatment did not affect femoral BVF compared to the respective control group (BVF/BW KO: 1.59, KO‐ABX: 1.49, n=7–11). Analysis of serum bone remodeling parameters indicated significantly lower serum osteocalcin levels (~ 37% of control levels) in WT‐ABX treated mice, while no changes were seen in the RAG‐KO group. Our study demonstrates that repopulation of the gut microbiota can be detrimental to the bone and that this effect is dependent on the T and B lymphocytes. Support or Funding Information Porter Fellowship awarded to Naiomy‐Deliz Rios Arce. National Institutes of Health Grants RO1 DK101050 and RO1 AT007695‐01 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .