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Veins Are Essential for Arteries: Immune Cells Implication
Author(s) -
Champin Tristan,
Grenier Céline,
Munier Mathilde,
Henrion Daniel,
Loufrani Laurent
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.899.11
Subject(s) - medicine , mesenteric arteries , inflammation , immune system , vein , artery , ligation , anatomy , pathology , immunology
Shear stress produces arterial diameter expansion associated with an hypertrophy induced by inflammation, macrophages, neutrophils or lymphocytes cells. Furthermore, arteries and veins are often associated through perivascular fat, allowing transfer of immune cells between both possible. So, we investigated the vein implication on immune cells recruitment for arterial remodeling of 12 weeks C57BL6/J mice. We produced various ligation models in mesenteric arteries or common carotids to produce High Flow Arteries (HFA). For each model, mesenteric or jugular veins associated with the HFA are ligatured or not. In resistance arteries, the absence of the vein associated with HFA, prevents HFA remodeling (HFA Ctrl: 320μm±27 vs HFA without vein: 218μm±19). Also, in conductance arteries, jugular ligation associated with HF carotid is able to prevents remodeling. qRT‐PCRs experiment have shown an increase of VCAM1 expression in veins during high‐flow mediated arteries remodeling. Vein ligation increase oxidative stress production. ROS generate a SIRT1 transcriptional activation leading to a decrease of VCAM1 expression. Immunostaining analysis, show that neutrophils and macrophages migrate from vein to artery. Immune cells are early recruited in vein but diapedesis modification is modulated by VCAM1 expression on vein endothelium. To conclude, we demonstrated for the first time that artery and vein interact each other by perivascular fat to generate mesenteric and conductance arteries remodeling. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .