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Sildenafil Abolishes Pulmonary Hypertension Induced by Left Heart Pressure Overload in Rats
Author(s) -
Chovanec Milan,
AlHiti Hikmet,
Kaftanova Barbora,
Durisova Jana,
Hampl Vaclav,
Herget Jan
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.892.17
Subject(s) - sildenafil , ventricle , medicine , pressure overload , cardiology , pulmonary hypertension , volume overload , heart failure , anesthesia , cardiac hypertrophy
Pulmonary hypertension (PH) induced by pressure overload of the left heart has recently attracted increasing attention in clinical practice. Unfortunately, no specific treatment of PH is approved for this group of patients. We previously described a simple rodent model of this condition. In this study, we used it to test a hypothesis that sildenafil can be beneficial in this type of PH. Left heart pressure overload was induced in adult male Wistar rats by partial intravascular obstruction of the ascending aorta. Three weeks later, sildenafil was given (25mg/kg, oesophageal gavage once a day) for the next 2 weeks (group HFS, n=7). The group HFS was compared to the group of rats with left heart pressure overload for 3 weeks with no additional use of sildenafil (group HF, n=9) and with controls (group C, n=6). Mean pulmonary arterial pressure was significantly lower in the group HFS compared to the group HF (12.9±0.8 mmHg vs. 20.3±1.0 mmHg, p<0.0001, respectively) but was not different from the group C (10.7±1.1 mmHg). Weight of the right ventricle relative to the body weight (RV/BW ratio) as well as right ventricle weight relative to the left ventricle plus septum (RV/LV+S ratio) were significantly lower in the group HFS compared to the group HF (RV/BW ratio: 4.1±0.3.10 −4 vs. 6.3±0.8.10 −4 ; p<0.01, respectively and RV/LV+S ratio: 0.23±0.02 vs. 0.41 ±0.07; p<0.05, respectively) but they were not different in the group HFS compared to the group C in both parameters. The amount of expired nitric oxide, measured by chemiluminescent method on day 10 of the left heart pressure overload, was significantly increased in the group HF compared to the group C (343.0±30.5 pg/min/100g vs. 239.8±18.2 pg/min/100g; p<0.05, respectively). Sildenafil abolishes the developed pulmonary hypertension caused by the left heart pressure overload in the rats. Support or Funding Information Supported by grants: GAUK 210216 and GACR 17‐11223S This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .