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Angiotensin 1–7 Action in the Lateral Preoptic Area is Involved in Urinary Bladder Regulation in Female Wistar Rats.
Author(s) -
Lamy Gustavo Bertollini,
Vale Barbara,
Almeida Roberto Lopes,
Brito Antonio Bruno,
Venancio Daniel Paulino,
Sato Monica Akemi
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.889.2
Subject(s) - medicine , hypertonic saline , endocrinology , angiotensin ii , urinary bladder , femoral artery , saline , isoflurane , blood pressure , anesthesia
Background Chemical lesions of the lateral preoptic area (LPA) enhance water intake induced by subcutaneous injection of hypertonic saline in rats. On the other hand, the LPA section abolishes the increase in intravesical pressure (IP) induced by olfactory tubercle stimulation in dogs. Immunohistochemical labeling has demonstrated the presence of Mas receptors for Angiotensin‐(1–7) in LPA. It is not known whether neurons with Mas receptors in LPA are involved in urinary bladder regulation. Aim We investigated the effects of activation or blockade of Mas receptors for angiotensin‐(1–7) in LPA on intravesical pressure in anesthetized female rats. Materials and methods Female Wistar rats (~240 g, N=6) underwent to a stereotaxic surgery for implantation of stainless steel guide cannulas into the LPA under ketamine and xylazin anesthesia. After 7 days, the animals were anesthetized and maintained with 2% isoflurane in 100% O 2 and submitted to cannulation of the femoral artery and vein, placement of a miniaturized Doppler flow probe around the left renal artery to measure the renal blood flow and cannulation of the urinary bladder for IP measurements. After baseline IP and cardiovascular parameters recordings for 15 min, angiotensin‐(1–7) (5 nmol/ uL) or saline (1 uL) or A‐779 (Mas receptor antagonist, 50 nmol/uL) was injected into the LPA and the variables were measured for additional 60 min. Results Unilateral injection of angiotensin‐(1–7) into LPA evoked a significant increase in IP (187.5±37.2%) compared to saline (−2.1±1.9%). Unilateral injection of A‐779 into LPA decreased the IP (−15.9±2.8%) in comparison to saline (2.0±1.1%). Interestingly, the decrease in IP was enhanced after bilateral injection of A‐779 into LPA (−27.3±3.4%) compared to the unilateral injection of this drug and also in comparison to saline (−2.9±1.6%) bilaterally into LPA. No significant changes in cardiovascular parameters after angiotensin‐(1–7) or saline or A‐779 in APL were observed. Conclusion The data suggest that LPA is an important part of the circuit that regulates the urinary bladder activity mediated by angiotensin‐(1–7). Support or Funding Information Financial support: FAPESP and NEPAS‐FMABC This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .