Premium
Calorie Restriction In The Preeclamptic‐like BPH/5 Mouse Reduces Adipose Tissue Gene Expression of Preeclampsia‐Related Inflammatory Genes
Author(s) -
Reijnders Dorien,
Olsson Kelsey,
Redman Leanne,
Sones Jennifer
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.882.9
Subject(s) - adipose tissue , preeclampsia , endocrinology , medicine , placenta , fetus , pregnancy , gene expression , intrauterine growth restriction , andrology , gene , biology , genetics , biochemistry
Background Preeclampsia (PE) is a hypertensive disease of pregnancy that is a leading cause of fetal and maternal morbidity/mortality. Obesity is a risk factor for developing PE, however, the underlying mechanism is unknown. Using the PE‐like BPH/5 mouse model, we previously demonstrated that cyclooxygenase (Cox)‐2 and interleukin (IL)‐15 dysregulation at the maternal‐fetal interface plays a causal role in poor maternal and fetal outcomes. Because BPH/5 mice are obese, we hypothesized that the maternal reproductive white adipose tissue (rWAT) is a source of these pro‐inflammatory molecules, and that reducing rWAT mass by calorie restriction (CR) would decrease the inflammatory uterine milieu in this model. Methods Using qRT‐PCR, we measured mRNA expression of Ptgs2 (the gene encoding Cox2), IL‐15 and 18S (housekeeper gene) within the rWAT and implantation sites of non pregnant and pregnant (embryonic day (e) 7.5) ad libitum fed BPH/5 and C57Bl/6J control mice. In addition, we determined Ptgs2 and IL‐15 mRNA expression in BPH/5 rWAT and implantation sites after 7 days CR (pair‐feeding with pregnant C57Bl/6J mice) beginning the day of copulation (e0.5). Results Ptgs2 expression in non‐pregnant rWAT was comparable between BPH/5 and C57Bl/6J. However, there was a 2‐fold increase in Ptgs2 expression at e7.5 in BPH/5 rWAT versus C57Bl/6J. After CR, Ptgs2 expression in BPH/5 rWAT was significantly lower compared to ad libitum fed BPH/5 mice (p=0.038, n=6). Also in implantation sites, the expression of Ptgs2 was significantly lower in CR as compared to ad libitum fed BPH/5 mice (p=0.046, n=6). IL‐15 expression was 30‐fold higher in BPH/5 rWAT and 8‐fold higher in implantation sites at e7.5 compared to C57Bl/6J control mice (p=0.0015, n=6), but this expression was not significantly affected by CR in either tissue. Conclusion These data show that 7 days CR in BPH/5 obese mice during early pregnancy reduces the expression of Cox‐2 coding genes in rWAT and at the maternal‐fetal interface, suggesting that WAT‐derived inflammatory mediators might be involved in the development of PE and poor pregnancy outcomes in obese BPH/5 mice. Future studies should investigate the molecular phenotype of WAT in women that are at risk to develop PE, to provide further insight in the mechanisms that make obesity a risk factor and possible interventions to prevent PE. Support or Funding Information This study has been funded by the Lousiana Biomedical Collaborative Research Program. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .