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Colonic Indole And Hydrogen Sulfide, Gut Bacterial Metabolites, Affect Portal Blood Pressure in Healthy And Cirrhotic Rats
Author(s) -
Ufnal Marcin,
Huc Tomasz,
Jurkowska Halina,
Wróbel Maria,
Jaworska Kinga,
Onyszkiewicz Maksymilian
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.873.3
Subject(s) - hemodynamics , medicine , endocrinology , cirrhosis , blood pressure , metabolite , thioacetamide , portal venous pressure , chemistry , portal hypertension
Liver cirrhosis is a leading cause of portal hypertension, and is often accompanied by systemic hypotension. Accumulating evidence suggests that gut bacteria may affect hemodynamics, however, the mechanisms are not clear. We investigated the effect of colon‐administered gut bacteria metabolites on portal blood pressure (PBP), portal blood flow (PBF) and systemic arterial blood pressure (ABP) in healthy (controls) and cirrhotic rats (CRH). 10‐week‐old, male, Sprague Dawley rats were maintained either on tap water (controls) or water solution of thioacetamide for 18 weeks to induce liver cirrhosis (CRH). PBP, PBF and ABP were measured in anaesthetized rats at baseline and after the intracolonic administration of either a vehicle, indole, or Na 2 S, a hydrogen sulfide (H 2 S) donor into the colon. CRH showed significantly higher PBP but lower ABP than controls. The intracolonic administration of indole increased portal blood level of indole, and peripheral blood level of indoxyl sulfate, a liver metabolite of indole. Rats treated with Na 2 S showed increased portal blood levels of thiosulfate and sulfane sulfur, products of H 2 S oxidation. The intracolonic administration of vehicle did not affect hemodynamic parameters. In controls intracolonic H 2 S increased PBP but decreased PBF and ABP. In contrast, intracolonic indole increased both BPB and PBF, whereas did not affect significantly ABP. In comparison to controls, CRH showed significantly greater hemodynamic responses to H 2 S but not to indole. Cirrhotic livers had a significantly higher concentration of H 2 S but lower expression of rhodanese, an enzyme converting H 2 S to sulfate. Colon‐administered indole and H 2 S increase portal blood pressure via distinct hemodynamic mechanisms. Indole increases, whereas H 2 S decreases portal blood flow. Indole and H 2 S signaling in the gut‐liver axis may be involved in the control of portal blood pressure and in the etiology of portal hypertension. Further studies on the effects of gut bacterial metabolites on portal circulation are needed. Support or Funding Information This work was supported by the National Science Centre, Poland grant no. UMO‐2016/22/E/NZ5/00647 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .