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L‐CaD alters cell spreading and adhesion force in osteoclasts
Author(s) -
Liou Yingming,
Chang ChuLung,
Chen JiannYeu,
Shih MingChih,
Wang ChihLueh Albert
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.869.1
Subject(s) - rankl , multinucleate , microbiology and biotechnology , chemistry , cell adhesion , bone resorption , cell fusion , cell , osteoclast , caldesmon , actin , gene silencing , integrin , biology , calcium , endocrinology , biochemistry , receptor , activator (genetics) , organic chemistry , gene , calmodulin
Non‐muscle caldesmon (l‐CaD) is known to participate in the regulation of actin cytoskeletal remodeling, but its function in osteoclastogenesis remains to be determined. In this study, a cell model was used to examine involvement of l‐CaD in the control of cell‐cell fusion during osteoclastogenesis by using gain‐ and loss‐of‐function in Raw264.7 cells followed by RANKL induction. In comparison with control cells, l‐CaD overexpression significantly increased TRAP activity, actin ring structure and bone resorption in RANKL‐induced cells. In contrast, gene silencing against l‐CaD decreased the potential for RANKL‐induced osteoclastogenesis and resorption function. To determine if l‐CaD is involved in the modulation of cell‐cell fusion in osteoclasts (OCs), atomic force microscopy (AFM) was used to resolve the mechanical changes of cell spreading and adhesion force in RANKL‐induced cells with l‐CaD overexpression and gene silencing. In addition, cell‐cell fusion was measured by the cell fusion index. Our data obtained showed that the expression level of l‐CaD is involved in the control of cell‐spreading and adhesion force that might facilitate cell‐cell fusion into multinucleate OCs during osteoclastogenesis. Support or Funding Information MOST‐106‐2320‐B‐005‐003‐MY2RANKL‐induced differentiation of RAW264.7 cells. (A)TRAP‐positive multinucleate OCs characterized with (B) actin ring formation around the cell periphery. (C)Actin ring structure is composed the core (labeled with l‐CaD) and the peripheral rim (labeled with vinculin).Effects of l‐CaD on RANKL‐induced (A)TRAP activity and (B) cell‐cell fusion.Studies using gain‐ and loss‐of‐function in Raw264.7 cells followed by RANKL induction showed that l‐CaD is involved in the regulation of (A)actin ring formation and (B)cell resorption.AFM used to determine the l‐CaD dependent changes of cell spreading in RANKL‐induced cells with overexpressing and gene silencing l‐CaD.Effect of l‐CaD on the adhesion force in RANKL‐induced cells with overexpressing and gene silencing l‐CaD.This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .