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Preventive effects of capillary regression on Brazilian honeybee propolis in disused skeletal muscle of rats
Author(s) -
Fujino Hidemi,
Kondo Hiroyo,
Matsumoto Tomohiro,
Ono Kohei,
Ikeji Takuya,
Takuwa Miho,
Hirabayashi Takumi,
Ishihara Akihiko
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.856.33
Subject(s) - hindlimb , skeletal muscle , endocrinology , soleus muscle , angiogenesis , medicine , tunel assay , chemistry , capillary action , anatomy , biology , immunohistochemistry , andrology , materials science , composite material
PURPOSE A chronic decrease in activity results in muscle capillary regression that is due, in part, to the overproduction of reactive oxygen species. Brazilian propolis (BP) is natural polyphenol‐rich substance collected by honeybees. The purpose of the present study was to investigate the protective effects of BP supplementation on capillary regression in hindlimb unloading‐induced atrophied soleus muscle. METHODS Twenty‐eight male Wistar rats were assigned randomly either to a control, control with BP supplementation, hindlimb unloaded or hindlimb unloaded with BP supplementation group for 14 days. The three‐dimensional capillary network of soleus muscle was visualized using a confocal laser scanning technique. The capillary volume, frequency distribution of luminal diameters and capillary‐to‐fiber ratio were measured. In addition, mojor proangiogenic factors, VEGF, Flt‐1, FlK‐1, anti‐angiogenic factors, thrombospondin‐1, and oxidative stress maker were determined. RESULTS The capillary volume, luminal diameter and capillary‐to‐fiber ratio in atrophied muscle were lower than those in control. BP supplementation, however, attenuated the capillary regression under disused muscle. In addition, the expression levels of thrombospondin‐1 and SOD‐1 were decreased and the level of VEGF was increased in BP supplemented muscle. Furthermore, TUNEL‐positive endothelial cells in BP supplemented muscle were decreased although TUNEL‐positive endothelial cells in atrophied muscle were increased. CONCLUSION These results suggest that Brazilian propolis supplementation may be an effective treatment to counter the regression of muscle capillary under disuse condition. Support or Funding Information Supported by Yamada Research Grant. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .