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The Impact of Chronic Doxorubicin Administration on Satellite Cell Population and Capillary Density in Multiple Skeletal Muscles
Author(s) -
D'Lugos Andrew C.,
Fry Christopher S.,
Hale Taben M.,
Gonzales Rayna J.,
Angadi Siddhartha S.,
Carroll Chad C.,
Dickinson Jared M.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.856.25
Subject(s) - doxorubicin , population , medicine , skeletal muscle , endocrinology , chemistry , urology , pathology , chemotherapy , environmental health
Anthracyclines are mainstay chemotherapeutic agents proven effective at reducing disease recurrence and mortality in breast cancer patients. However, these drugs also have harmful side effects that include skeletal muscle wasting. The purpose of this study was to assess the impact of bi‐weekly doxorubicin (DOX) administration on the satellite cell population and capillary density in multiple skeletal muscles. We hypothesized that DOX treatment would reduce fiber size, along with the satellite cell population and capillary density of the soleus (SOL) and extensor digitorum longus (EDL) muscles. Eight‐week old ovariectomized female Sprague‐Dawley rats were randomized to one of two treatments: DOX and vehicle (Veh). DOX (4 mg/kg) or Veh (saline) intraperitoneal injections were performed bi‐weekly for a total of 3 injections (cumulative dose 12 mg/kg). Animals were euthanized 5d following the last injection, during which the SOL and EDL were dissected and prepared for immunohistochemical analysis. Relative to Veh, cross‐sectional area (CSA) of the SOL and EDL muscle fibers were 30% and 45% smaller, respectively, in DOX (p<0.05 vs. Veh). The satellite cell population and capillary density were 40% and 35% lower, respectively, in the SOL of DOX‐treated versus Veh‐treated rats (p<0.05), whereas the satellite cell population and capillary density in the EDL were unchanged by DOX (p>0.05). Chronic doxorubicin administration is associated with reduced fiber size in multiple skeletal muscles, however doxorubicin appears to impact the satellite cell population and capillary density in a muscle‐specific manner. Support or Funding Information Intramural funds from Arizona State University. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .