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Prospective Study To Investigate The Prevalence Of The 90 kDa Isoform Of Angiotensin Converting Enzyme In Vitória ‐ Brazil
Author(s) -
Gomes Andreia Cristina,
Fernandes Fernanda Barrinha,
Carmo Franco Maria,
Mill Jose Geraldo Carmo,
Casarini Dulce Elena
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.847.5
Subject(s) - gene isoform , renin–angiotensin system , incidence (geometry) , angiotensin converting enzyme , medicine , population , prospective cohort study , endocrinology , enzyme , blood pressure , biology , biochemistry , environmental health , gene , physics , optics
Hypertension is considered a worldwide public health problem and identified as a risk factor for cardiovascular morbidity and mortality. The Renin Angiotensin System (RAS) has been a focus of interest of researchers in the area of hypertension both for the identification of its etiopathogenesis and for its treatment. In order to identify the components involved in the onset of hypertension and the participation of Angiotensin I Converting Enzyme (ACE) in this problem, the RAS has been the object of many studies. ACE has a great physiological importance for converting angiotensin I (Ang I) to angiotensin II (Ang II), a potent vasoconstrictor. Some papers suggest the association of ACE isoforms, especially the 90 kDa isoform, with hypertension. The aim of this study was to investigate the association between ACE isoforms and the presence of arterial hypertension in the second phase of the prospective study (MONICA Project) in the population of Vitória ‐ ES, Brazil. Methods The demographic, clinical and biochemical parameters of 220 individuals were evaluated. Urine samples were concentrated and ACE isoforms were identified by the Western Blotting technique. Results The groups were classified as Group 1 (presence of isoforms with 65, 90 and 190 KDa); Group 2 (presence of 65 and 90 kDa isoforms) and Group 3 (presence of 65 and 190 kDa isoforms). The results showed a high prevalence of the 90 kDa isoform with a higher incidence of hypertension (higher in group 2) after five years of segment. The groups containing 90 kDa isoform showed higher systolic and diastolic pressure profiles in the second phase and the frequency of normotensives expressing the isoforms with 65 and 190 kDa was higher than that the hypertensive ones. Loss of isoform expression at 190 kDa increases the chances of developing hypertension as well as family history of hypertension. Conclusion The results suggest the 90‐kDa N‐domain isoform as a possible biological marker of hypertension, which confirms the results obtained in the first phase of the study. Support or Funding Information CNPq and FAPESP This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .