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Measurement of Electrolytes, Including Acetate in Various Physiological Samples Using Ion Chromatography
Author(s) -
Chapp Andrew D.,
Schum Simeon,
Behnke Jessica E.,
Huber Michael J.,
Jiang Enshe,
Larson Robert A.,
Shan Zhiying,
Chen QingHui
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.844.3
Subject(s) - chemistry , ion chromatography , sodium , potassium , chloride , chromatography , ammonium acetate , ammonium chloride , electrolyte , ammonium , ion , magnesium , calcium , nuclear chemistry , high performance liquid chromatography , electrode , organic chemistry
Accurate quantification of cations and anions remains a major diagnostic tool in understanding diseased states. The current technologies used for these analyses are either unable to quantify all ions due to sample size/volume, instrument setup/method, or are only able to measure ion concentrations from one physiological sample (liquid or solid). Herein, we adapted a common analytical chemistry technique, ion chromatography and applied it to measure the concentration of cations; sodium, potassium, calcium, magnesium and ammonium (Na + , K + , Ca 2+ , Mg 2+ , NH 4 + ) and anions; chloride and acetate (Cl − , − OAc) from physiological samples. Specifically, cations and anions were measured in liquid samples: serum and cerebrospinal fluid, as well as tissue samples: liver, cortex, hypothalamus and amygdala. Serum concentrations of Na + , K + , Ca 2+ , Mg 2+ , Cl − , and − OAc (mM): 138.8 ± 4.56, 4.05 ± 0.21, 4.07 ± 0.26, 0.98 ± 0.05, 97.7 ± 3.42 and 0.23 ± 0.04, respectively. Cerebrospinal fluid concentrations of Na + , K + , Ca 2+ , Mg 2+ , Cl − , and − OAc (mM): 145.1 ± 2.81, 2.41 ± 0.26, 2.18 ± 0.38, 1.04 ± 0.11, 120.2 ± 3.75, 0.21 ± 0.05, respectively. Ammonium was undetectable in serum and cerebrospinal fluid. Tissue Na + , K + , Ca 2+ , Mg 2+ , NH 4 + , Cl − and − OAc were also measured. Validation of the ion chromatography method was established by comparing chloride concentration between ion chromatography with a known method using an ion selective chloride electrode. These results indicate that ion chromatography is a suitable method for the measurement of cations and anions, including acetate from various physiological samples. Support or Funding Information Funding support: AHA 16PRE27780121, Andrew Chapp, R15‐HL129213, Zhiying Shan; R15‐HL122952, Qing‐Hui Chen. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .