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Anti‐inflammatory effect of a new formulation: Palmitoylethanolamide/Baicalein in a murine model of Myocardial Ischaemia/Reperfusion Injury
Author(s) -
Cuzzocrea Salvatore,
D'Amico Ramona,
Fusco Roberta,
Gugliandolo Enrico,
Crupi Rosalia,
Impellizzeri Daniela,
Di Paola Rosanna
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.841.9
Subject(s) - baicalein , pharmacology , oxidative stress , palmitoylethanolamide , medicine , reperfusion injury , nitrotyrosine , ischemia , degranulation , chemistry , nitric oxide , receptor , nitric oxide synthase , cannabinoid receptor , agonist
Myocardial ischemia/reperfusion (I/R) injury is the principal cause of death, happens after prolonged occlusion of the coronary arteries. The first intervention to limit myocardial damage is directed toward restoration of perfusion as soon as possible, however infarction triggers a powerful inflammatory response and a significant oxidative stress. Palmitoylethanolamide (PEA), is a well‐known fatty acid amide‐signaling molecule that possess an important anti‐inflammatory and analgesic effects. Nevertheless, PEA does not possess the ability to prevent free radicals formation. Baicalein, a bioactive component isolated from a Chinese herbal medicine, has multiple pharmacological activities, such as a strong anti‐oxidative effects. A combination of PEA and Baicalein could have beneficial effects on oxidative stress produced by inflammatory response. In the present study we investigated the effects of composite containing PEA and Baicalein in a model of myocardial I/R injury. Myocardial ischemia/reperfusion injury was induced by occlusion of the left anterior descending coronary artery for 30 min followed by 2 h of reperfusion. PEA‐Baicalein (10:1), was administered (10 mg/kg) 5 min before the end of ischemia and 1 h after reperfusion. In this study, we clearly demonstrated that PEA‐Baicalein treatment reduces myocardial tissue injury, neutrophils infiltration, markers for mast cell activation expression such as chymase and tryptase and pro‐inflammatory cytokines production (TNF‐α, IL‐1β). Moreover, PEA‐Baicalein treatment reduces nitrotyrosine and PAR formation, inhibits NF‐kB nuclear translocation and modulates apoptosis pathways. Accordingly, these results support the idea that the association between PEA and Baicalein should be further studied as a potent candidate for the treatment of myocardial I/R injury. Support or Funding Information no funding This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .