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Effect of Berberine on Insulin, GLP‐1R and DPP4 Gene Expression Levels in Streptozotocin Induced Diabetic Rats
Author(s) -
Kilari Eswar Kumar,
Swathi P
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.838.7
Subject(s) - berberine , medicine , streptozotocin , endocrinology , diabetes mellitus , insulin , chemistry , pharmacology
Berberine is a quaternary ammonium compound belongs to benzylisoquinoline alkaloids . Traditionally it was used in the treatment of diabetes and associated complications. The aim of the present study was to determine the effect of berberine on antihyperglycemia, in vivo antioxidant, hepatic and lipid biomarkers and gene expressions in streptozotocin induced diabetic rats. Albino Wistar rats were induced for diabetes by streptozotocin and divided into five groups as vehicle control (group I), disease control (group II), standard gliclazide (Group III), group IV, and V received Berberine 50 and 100 mg/kg body weight respectively orally once a day for 15 days in STZ induced diabetic rats. A significant increase in blood glucose, glycosylated hemoglobin (HbA1c), altered Lipid profile (Total cholesterol, triglycerides, HDL, LDL and VLDL) and elevated hepatic serum biomarker levels (AST, ALT, ALP, total & direct bilirubin) in STZ induced diabetic rats. The berberine showed significant reduction of blood glucose and HbA1c and elevation of insulin levels in STZ induced diabetic rats. The Berberine shown to normalize the lipid and hepatic biomarkers during 15 days treatment period in all groups of treatments in a dose dependent manner in STZ induced diabetic rats. Decreased levels of in vivo antioxidant enzymes SOD, CAT, GSH and GPx in both pancreas and ileum, decreased insulin & GLP‐1R and increased hepatic DPP4 mRNA gene expression levels in STZ induced diabetic rats. Treatment with Berberine shown to elevate the pancreatic and intestinal antioxidant enzymes and increased pancreatic insulin & GLP‐1R gene expression in ileum and decreased hepatic DPP4 in STZ induced diabetic rats. The pharmacological activity of the berberine might be due to the antioxidant property and by enhancing the incretin activity. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .