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Inhibition of STAT3 phosphorylation by curcumin partly reduces adhesion molecule expression in vascular endothelial cell
Author(s) -
Ahn Hee Yul,
Cho Young Suk,
Sankaralingam Sowndramalingam
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.837.6
Subject(s) - curcumin , cell adhesion molecule , cell adhesion , stat3 , chemistry , vcam 1 , phosphorylation , endothelial stem cell , microbiology and biotechnology , lipopolysaccharide , monocyte , western blot , umbilical vein , pharmacology , biology , icam 1 , cell , biochemistry , immunology , in vitro , gene
OBJECTIVES We investigated whether curcumin, an ingredient of turmeric, can suppress the expression of intercellular adhesion molecule 1 (ICAM‐1) and vascular cell adhesion molecule 1 (VCAM‐1) in human umbilical vein endothelial cells (HUVEC) stimulated with lipopolysaccharide (LPS), and the underlying mechanisms. MATERIALS AND METHODOLOGY The levels of adhesion molecules, activities of nuclear factor kappaB (NF‐kB) and signal transducer and activator of transcription 3 (STAT3) were determined using Western blot analysis. The effect of curcumin on monocyte binding to endothelial cells was determined using THP‐1 monocyte (human acute monocytic leukemia cell line) adhesion assay. RESULTS Curcumin prevented the LPS‐mediated increase in ICAM‐1 and VCAM‐1 expression in HUVEC in a dose‐dependent manner. Curcumin did not affect LPS induced degradation of IkappaBα (IkBα). On the other hand, curcumin reduced the LPS‐induced STAT3 phosphorylation. Curcumin significantly reduced LPS‐mediated THP‐1 monocyte adhesion to HUVEC. Furthermore, in C57BL/6 mice, curcumin prevented the LPS induced increase in aortic ICAM‐1 and VCAM‐1 expression. CONCLUSIONS Curcumin prevented LPS‐induced ICAM–1 and VCAM–1 expression on vascular endothelial cells associated with inhibition of STAT3 phosphorylation. Support or Funding Information This work was supported by the research grants of the Chungbuk National University in 2014. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .