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Regulation of Hydrogen Sulfide Levels by Cannabinoids in the Bovine Isolated Retina
Author(s) -
Bush Leah,
Robinson Jenaye,
Kutse Seun,
Ebuzoeme Christabel,
Onyenaka Collins,
Akpan Idongesit,
Opere Catherine,
Ohia Sunny,
NjieMbye Ya Fatou
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.825.12
Subject(s) - neuroprotection , chemistry , methylene blue , endocannabinoid system , retina , incubation , retinal , biochemistry , cannabinoid , ethanol , pharmacology , biology , catalysis , neuroscience , receptor , photocatalysis
Both endocannabinoids and hydrogen sulfide (H 2 S) have been reported to exert neuroprotective actions in neural tissues ( Biermann et al., Mol Vis. 2011;17:1275–86; Nucci et al., Invest Ophthalmol Vis Sci. 2007;48(7):2997–3004 ). It remains to be determined if these modulators can interact with each other and/or share a common pathway in their neuroprotective actions. Purpose The aim of the present study was, therefore, to investigate the pharmacological actions of endocannabinoids on the biosynthesis of H 2 S in isolated bovine neural retina. Methods Isolated bovine retinae were homogenized and exposed to different concentrations of methanandamide (1 nM – 10 uM) and 2‐arachidonyl glycerol (2‐AG) (1 – 3 uM) for 90 minutes at 37ºC. Retinal homogenates were then incubated with ethylenediaminetetraacetic acid (EDTA) (1%), Zinc acetate (1%), and Borate buffer (pH 11) for 30 mins followed by 10‐minute incubation with N, N dimethyl p‐phenylenediamine (20 mM) and FeCl 3 (300 mM). Samples were then centrifuged at 5000 g for three minutes and supernatant was assessed for H 2 S content using the well‐established Methylene Blue method. Results Both methanandamide and 2‐AG elicited a concentration‐dependent decrease in basal H 2 S production in the isolated retina. For instance, methanandamide (10 μM) significantly (p <0.05) reduced basal production of H 2 S by 30% when compared to controls. Likewise, 2‐AG (10 μM) also elicited a 30% decrease in basal retinal H 2 S concentration when compared to controls. Conclusions We conclude that exogenously applied endocannabinoids can alter the basal production of H 2 S in the isolated neural retina. The observed effect of endocannabinoids on the pathway leading to H 2 S biosynthesis suggests that a cross‐talk exists between both neuromodulators in the retina. Support or Funding Information Supported by NIH/NEI Research Grant 1R15EY022215‐01 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .