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Protein Expression from Measles (MV) L Protein Genes Optimized and Suboptimized to Human and Canine Codon Usage Bias Varies with Construct and Cell Type
Author(s) -
Shah Akash,
Uhl Elizabeth W.,
Osborn Michelle L.,
Michel Frank,
Hogan Robert J.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.819.20
Subject(s) - transfection , gene , microbiology and biotechnology , biology , gene expression , codon usage bias , hek 293 cells , messenger rna , genetics , genome
The purpose of this study was to characterize protein expression from constructs of the MV polymerase (L) gene optimized to human and canine codon usage bias (CUB). All codon changes made were synonymous in that the amino acid coded for was unchanged. Four constructs of the MV L gene were transfected into human (HEK 293) and canine (A‐72) cells: 1) human CUB optimized, 2) canine CUB optimized, 3) human CUB sub‐optimized, and 4) wild type (WT). Protein expression from transfected cells was assessed using fluorescent labeling and Cellomic analysis. Human cells transfected with constructs of the MV L gene optimized to human or canine CUB had increased expression compared to cells containing WT or sub‐optimized constructs. In contrast there was no difference in the levels of protein expression in canine cells transfected with the MV L gene constructs. These results complete analysis of the effects of human and canine CUB optimization on protein expression from MV genes. Overall these studies indicate that synonymous codon changes in viral nucleic acid sequences can alter protein expression in a variety of ways, some of which are likely to impact viral pathogenicity. Support or Funding Information The project depicted was sponsored by the Department of Defense Threat Reduction Agency. The content of the information does not necessarily reflect the position or the policy of the federal government, and no official endorsement should be inferred. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .