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Characterization of a Short Form of Perilipin 5
Author(s) -
Dalton Rebekah C.,
Tansey John T.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.814.8
Subject(s) - perilipin , lipid droplet , lipid metabolism , biochemistry , biology , microbiology and biotechnology , chemistry , lipolysis , adipose tissue
Many modern health issues arise from aberrant lipid metabolism. Among these are type II diabetes and non‐alcoholic fatty liver disease. One commonality these diseases share is neutral lipid metabolism and storage. Perilipins are a family of five conserved proteins that are found on the surface of lipid storage droplets and play a central role in the regulation of cellular neutral lipid metabolism. Perilipin 5 is expressed in tissues with a high capacity for fatty acid oxidation such as heart, oxidative muscle, and fasting liver. Using western blotting and reverse transcriptase PCR we have identified a shortened form of perilipin 5 we have termed perilipin 5B. This short form of the protein retains the amino terminal 35 kDa of the protein but lacks the 4 helix bundle found in the C‐terminus. Based on reports in the literature, this protein would lack the carboxy terminal domains reported to be necessary for interactions with lipases and other proteins but would retain serine 155 that is requisite for PKA phosphorylation and translocation to the nucleus. We have constructed a carboxy terminal truncation of perilipin 5 and are expressing it in CHO cells to further define the function of this protein. Like the full length protein, this truncation targets to lipid storage droplets. Collectively these studies indicate that perilipin 5B plays a role in lipid storage, but its function is still unknown. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .