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Modulation Of Cellulase Activity Of Fungi Isolates For Bioethanol Production By Using Cassava Bagasse
Author(s) -
IgwoEzikpe Miriam Nwanna,
Okunowo Wahab Oluwanisola,
Ayanshina Oluwamuyiwa
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.814.3
Subject(s) - cellulase , bagasse , food science , biofuel , chemistry , enzyme assay , trichoderma , biomass (ecology) , fermentation , microbiology and biotechnology , enzyme , biology , biochemistry , agronomy
The continual energy demand worldwide and the progressive demising of oil reserves motivate the search for alternative energy resources especially from renewable sources such as agrowaste biomass. This study investigated the optimization of cellulase activity for bioethanol production through modulation of the cellulase activity of three fungi isolated from cassava bagasse. Fungi were isolated from cassava bagasse and genetically identified using 16S rDNA. The isolates were screened for cellulase enzyme production by filter paper and carboxymethylcellulase (CMC) assay. Physiochemical factors (Temperature, moisture content, nitrogen source, pH, substrate and inoculum concentration) which affect the cellulase activity were evaluated. Three fungi isolates were identified as Trichoderma Spp, Aspergillus spp and Rhizopus spp. The exoglucanase and endoglucanase had optimum activity at 25°C and 35°C, moisture content effect at 40 ml, pH 7, inoculum concentration of 10 – 20 ml and substrate concentration of 10–15g. Peptone greatly enhanced enzyme production better than urea and ammonium sulfate at day 3 incubation for both the exoglucanase and endoglucanase. Present study established the potential of these fungi isolates from agrowaste as good cellulolytic enzyme for potential use in bioethanol production. Support or Funding Information TETFUND Research Grant Award, 2015 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .