Premium
Improvement of joint health by anti‐inflammatory activity of natural substances in chondrocyte of osteoarthritis
Author(s) -
Kim Dakyung,
Lee Minhee,
Park SooJeung,
Yun JeongMoon,
Lee Dasom,
Sohn Johann,
Yun MinKyu,
Lee Jeongmin
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.812.46
Subject(s) - osteoarthritis , aggrecan , chondrocyte , inflammation , cartilage , medicine , tumor necrosis factor alpha , immunology , type ii collagen , matrix metalloproteinase , pharmacology , pathology , articular cartilage , anatomy , alternative medicine
Osteoarthritis (OA) is a chronic degenerative disease that involves inflammation and pain due to damage or degenerative changes in the cartilage that protects the joints. OA has a high incidence in the elderly. Treatment methods include relieving symptoms and preventing cartilage damage. Drugs used to treat osteoarthritis have side effects and it is important to reduce them. Therefore, it is necessary to develop a therapeutic agent for osteoarthritis using natural substances. The purpose of this experiment was the anti‐inflammation effect of natural substances on osteoarthritis in primary cultured rat cartilage chondrocytes. Aggrecan, collagen type I, □, □, MMP‐3, 7, NK‐kB and COX‐2 expression were measured by real‐time PCR. TNF‐α and IL‐1β secretion was measured by ELISA kit. H 2 O 2 and LPS‐induced chondrocytes were used to measure concentrations of 100, 200, and 400 (μg/ml). As a result, S400 significantly increased the gene expression level of aggrecan, collagen type I, II, III and decreased gene expression of pro‐inflammatory factors NF‐kB, COX‐2 and MMPs (3 and 7). The cytokine productions of TNF‐α and IL‐1β also decreased in S400. Therefore, S400 had an anti‐inflammatory effect and alleviated the inflammation occurs in OA. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .