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Investigating the regulation of intercellular trafficking in plants with varying levels of thioglucoside glucohydrolase mutants
Author(s) -
Fernandez Jessica Cisneros,
Hewezi Tarek,
BurchSmith Tessa
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.808.9
Subject(s) - plasmodesma , glucosinolate , mutant , myrosinase , nicotiana benthamiana , arabidopsis thaliana , microbiology and biotechnology , intracellular , biology , arabidopsis , biochemistry , wild type , gene , cytoplasm , botany , brassica
Plants have evolved cell‐wall pores called plasmodesmata to allow direct communication between connected cells. Plasmodesmata facilitate the intercellular trafficking of nutrients, metabolites and signaling molecules including hormones, small RNAs, and proteins. In the Arabidopsis thaliana increased size exclusion limit 2 (ise2) mutant, there is increased intercellular trafficking and increased numbers of branched plasmodesmata. Similar changes have been observed in ISE2‐ silenced Nicotiana benthamiana leaves. In an effort to identify proteins that interact with ISE2, a pull‐down assay with purified MBP‐ISE2 as bait followed by protein identification by mass spectrometry was performed. THIOGLUCOSIDE GLUCOHYDROLASE 2 (TGG2) emerged as a potential ISE2‐interacting protein. In a separate gene expression analysis, expression of TGG1 was significantly decreased in ise2 mutant embryos compared to wild‐type controls. TGGs are myrosinases, enzymes responsible for catalyzing the hydrolysis of glucosinolates to unstable aglycons, which are re‐arranged to form toxic defense compounds like isothiocyanates. In A. thaliana , TGG1 and TGG2 are duplicate genes expressed in above‐ground tissues. To investigate the relationship between ISE2 and TGG1 and/or TGG2, we monitored expression of genes in the glucosinolate pathway in plants with varying levels of ISE2 expression. We also measured intercellular trafficking in mutants of the glucosinolate pathway. Preliminary data suggest that overexpression of TGG1 increased nematode susceptibility in two lines and two mutant lines, tgg1–1 and tgg1–2 , showed no significant difference from the wild‐type. Further, glucosinolate levels and intercellular trafficking were measured in plants overexpressing myrosinase TGG1. The results suggest that glucosinolates could play a role in mediating ISE2's effect on intercellular trafficking, particularly during plant defense. Support or Funding Information TBS supported by National Science Foundation Award 1456761 and start‐up funds from the University of Tennessee This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .