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Identification and Functional Validation of a Biomarker for the Diagnosis of Miltefosine relapse during Visceral Leishmaniasis
Author(s) -
TIWARY PUJA,
KUMAR DINESH,
Sundar Shyam
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.807.3
Subject(s) - miltefosine , biomarker , calpain , cysteine protease , biology , blot , visceral leishmaniasis , leishmaniasis , immunology , protease , pharmacology , medicine , microbiology and biotechnology , gene , genetics , biochemistry , enzyme
Miltefosine is the only orally administrable drug for the treatment of leishmaniasis. But in recent years, a decline in its efficacy points toward the emergence of resistance to this drug. Knowledge of biomarkers for miltefosine resistance may be beneficial for proper selection of treatment regimen. Splenic aspirates were collected and parasites cultured from patients relapsed after initial cure (n=15) and successfully treated (n=15) with miltefosine. Differential expression of genes in miltefosine resistant strains, was examined by DNA microarray, and validated by real time RT‐PCR and western blotting. Of 669 up‐regulated genes, the cysteine protease‐like protein of calpain family [GenBank: CBZ34784] was found significantly over expressed in resistant parasite strains and only anti calpain antibodies showed its presence in the sera of relapse patients through western blotting. Calpain family cysteine protease‐like protein can be useful as potential biomarker of miltefosine unresponsiveness. Support or Funding Information This research did not receive any specific grant from funding agencies in the public, commercial, or not‐for‐profit sectorsCloning, expression and immunoblotting of recombinant Calpain family cysteine protease‐like protein and hypothetical protein 2This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .