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Quantifying HNF1A Regulated Gene Expression in Pancreatic Cancer
Author(s) -
Alaniz Anthony A.,
Abel Ethan V.,
Simeone Diane M.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.804.43
Subject(s) - hnf1a , pancreatic cancer , gene knockdown , hepatocyte nuclear factors , cancer research , downregulation and upregulation , biology , cancer , microarray analysis techniques , transcription factor , gene , gene expression , medicine , genetics
Hepatocyte nuclear factor 1A (HNF1A) is a transcription factor essential to normal pancreas and liver development and homeostasis. HNF1A has been shown to be upregulated in pancreatic cancer stem cells (CSC), a proposed key driver of pancreatic ductal adenocarcinoma (PDAC), relative to other pancreatic cancer cells. A CSC gene signature consisting of 50 upregulated genes, including HNF1A, was previously identified by a microarray. This signature predicts worse survival compared to HNF1A expression alone. This research aims to establish an HNF1A‐dependent gene signature that could potentially be used as a biomarker for pancreatic cancer. Using quantitative reverse transcriptase PCR, multiple CSC signature genes were shown to respond to HNF1A knockdown and overexpression. Responsive genes varied across cell lines, suggesting a complex pathway significantly affected by HNF1A transcriptional control. Support or Funding Information The work was supported by the Pancreatic Cancer Action Network‐AACR Pathway to Leadership Grant (16‐70‐25‐ABEL) and the American Cancer Society Postdoctoral Fellowship (127662‐PF‐15‐033‐01‐DDC) (to EVA) and the Gershenson Pancreatic Cancer Fund (DMS) and SKY Foundation (HC and DMS). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .