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Real Time PCR analysis of Ewing's Sarcoma Cell Lines in Response to Chemotherapeutic Treatment.
Author(s) -
Pitzen Sam,
Kruchten Anne
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.804.37
Subject(s) - etoposide , sarcoma , ewing's sarcoma , cd99 , cancer research , medicine , fli1 , metastasis , cancer , chemotherapy , oncology , biology , pathology , gene , chromosomal translocation , immunohistochemistry , vimentin , biochemistry
Ewing's Sarcoma is a rare bone and soft tissue cancer typically found in children ages ten to twenty years old. Survival rate is about 70% if caught before metastasis, but drops to 30% if the cancer has metastasized throughout the body. Treatment options for Ewing's Sarcoma include amputation, radiation, and/or chemotherapy. To better understand the effects of chemotherapeutic drugs on cultured Ewing's Sarcoma cell lines, we treated Ewing's cells with etoposide, one of the common drugs of the chemotherapeutic cocktail treatment for Ewing's, and with tamoxifen. We used real‐time PCR to analyze mRNA levels of several targets in cells treated with these various chemotherapies. One gene target is GLI1, which is highly expressed in primary tumors. Another target gene sequence is TP53, which is normally a tumor suppressor. mRNA was collected from cells treated with the various chemotherapies andy multiple targets were analyzed. Here we report the results of real time PCR using Taqman probes. Our results contribute to our understanding of how chemotherapies may effect Ewing's Sarcoma tumors and future cultured cell line research in this area. Support or Funding Information This work is funded by the College of Saint Scholastica SSC Summer Research Fellowships. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .