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The Role of the Vacuolar (H+)‐ATPase in Neuroblastoma Cell Differentiation induced by microRNA 506‐3p
Author(s) -
Medrano Geraldo,
Zhao Zhenze,
Du Liqin
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.804.3
Subject(s) - neuroblastoma , neural crest , microrna , atpase , cancer research , small interfering rna , biology , gene knockdown , pediatric cancer , suppressor , microbiology and biotechnology , cancer , cell growth , cellular differentiation , gene , embryo , rna , genetics , cell culture , biochemistry , enzyme
Neuroblastoma is one leading causes of cancer‐related childhood deaths. Neuroblastoma arises from the developing sympathetic nervous system where neural crest cells fail to complete the differentiation process. The failure in the differentiation process initiated the investigation into differentiation‐inducing agents. MicroRNA 506‐3p (miRNA‐506) was previously identified to be a potent tumor suppressor and induced neuroblastoma cell differentiation. A gene expression array determined that the ATP‐driven proton pump, vacuolar (H+)‐ATPase (V‐ATPase) is directly targeted by the miRNA‐506. However, the role of V‐ATPase in neuroblastoma has not been investigated. We recently found that by using siRNAs to knock down V‐ATPase expression we were able to reduce neuroblastoma proliferation and cell viability. These results warrant further investigation to determine the molecular mechanisms through which V‐ATPase helps mediate the effects of miRNA‐506 on neuroblastoma cells. Support or Funding Information The authors would like to thank the support for this project provided in part by the DoD, Texas State University, and South Texas Doctoral Bridge Program (GM102783) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .