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Towards independent cellular release of a multi‐biomolecule system using gold nanorods: Simultaneous up and down regulation of cellular pathways with light control
Author(s) -
Grossman Shan,
Morgan Erin,
Reich Norbert
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.801.7
Subject(s) - nanorod , fluorescence , biomolecule , biophysics , chemistry , nanotechnology , irradiation , laser , materials science , physics , nuclear physics , optics , quantum mechanics , biology
The control of cellular pathways using light has enormous potential to improve our understanding of biological processes for basic science and biomedical applications. No such technology is currently available to simultaneously up and down regulate multiple inputs. We have developed a robust gold nanorod platform that allows the controlled delivery of regulatory proteins and nucleic acids, relying on benign near infrared light exposure. Gold nanorods (GNR) are used because they absorb in the NIR region, they can release their cargo through breaking gold thiol bonds, and are capable of endosomal release. GNR that have peak absorbances at ~750 nm (GNR 750 ) and ~1000 nm (GNR 1000 ) were synthesized and functionalized with double stranded DNA via a gold‐thiol bond. GNR were irradiated with 800 nm pulsed light for 15 seconds, then separate samples were irradiated with 1150 nm pulsed light for 15 seconds. DNA release was quantified using TECAN fluorescence intensity assay and compared against controls. GNR 750 showed ~50% release as expected when irradiated with 800 nm pulsed laser, and negligible release when irradiated with 1150 nm pulsed laser. GNR 1000 showed the same release patterns after adjusting laser settings to lower energy/pulse at 730 nm. These results show that we have the ability to precisely release each cargo specifically at a single wavelength and not release it at another. We are currently working on using this platform to induce an “OFF‐ON‐OFF” sequence of red fluorescent protein expression in a mutant reporter cell line through the delivery of siRNA and Cre Recombinase protein. This proof of concept will move the project towards our long term goal of providing a novel method to control cellular events, thereby enabling fundamental interrogation in ways not presently achievable. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .