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Determination of Iron in Biological Samples using Sector‐Field vs. Quadrupole Inductively Coupled Plasma Mass Spectrometry
Author(s) -
ALOgaidi Hussein A.,
Ketterer Michael E.,
Ragan Emily J.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.800.8
Subject(s) - chemistry , inductively coupled plasma mass spectrometry , polyatomic ion , mass spectrometry , analytical chemistry (journal) , detection limit , quadrupole , isotope , isotope dilution , inductively coupled plasma , ion , chromatography , plasma , atomic physics , physics , organic chemistry , quantum mechanics
Inductively coupled plasma mass spectrometry (ICPMS) has become the preferred technique for trace and ultra‐trace determination of elemental concentrations in a wide variety of sample matrices. In our studies of iron (Fe) uptake and metabolism in insect cells, we evaluate different ICPMS procedures to measure pg – ng quantities of Fe in whole insects and Drosophila melanogaster S2 cells (Sg4 isolate); samples are digested and mineralized by heating with nitric acid (HNO 3 ) at 90°C in fluoropolymer containers. The determination of Fe by ICPMS is well‐known to be affected by interferences from polyatomic ions; e.g., the species 40 Ar 16 O + forms in the plasma and directly impacts the most abundant 56 Fe isotope. We evaluate two ICPMS approaches for performing reliable low‐level Fe determinations: A) quadrupole ICPMS with a collision cell system for attenuation of polyatomic ions; and B) a sector field ICPMS system capable of producing high resolving powers (m/Δm ~ 10,000). The latter system is easily capable of physically separating the 56 Fe + peak (55.94 amu) from interfering 40 Ar 16 O + (55.96 amu), and can generate solution detection limits of ~ 0.01 mg L −1 , which is far superior to the ~ 1 mg L −1 detection limits attainable by quadrupole ICPMS with a collision cell in the kinetic energy discrimination mode. We describe the relative merits of using internal standardization with 59 Co vs. isotope dilution with a 57 Fe‐enriched spike solution. Standard operating procedures for the determination of Fe using SF‐ICPMS will be discussed. Support or Funding Information NSF grant IOS‐1656407 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .