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Towards the Evaluation of Quorum Sensing Signal Synthase Inhibitors
Author(s) -
Higgins Erin Leigh,
Payne Sterling,
Ulrich Scott
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.796.11
Subject(s) - autoinducer , quorum sensing , biofilm , bacteria , gene , pathogenic bacteria , biology , virulence , microbiology and biotechnology , chemistry , biochemistry , computational biology , genetics
Quorum sensing is a system through which bacteria sense and respond to their cell density by altering their gene expression. This behavior is executed through the synthesis, secretion, and detection of a class of small molecules called autoinducers. Some bacteria that are clinically relevant human pathogens contain a quorum sensing system that controls genes related to virulence and biofilm formation. As such, quorum sensing represents a reasonable antibacterial drug target for the control of pathogenic behavior without targeting bacterial growth or survival. Acyl‐homoserine lactones, the autoinducer for gram‐negative bacteria, is catalyzed by the LuxI family of autoinducer synthases. This project involves the expression, purification, and kinetic characterization of the LuxI family of autoinducer synthases and design of synthase inhibitors to block quorum‐sensing induced gene expression. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .