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TMED2 is Required in both the Chorion and Placenta for Placental Labyrinth Layer Development
Author(s) -
JeromeMajewska Loydie,
Hou Wenyang
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.779.4
Subject(s) - biology , microbiology and biotechnology , golgi apparatus , endoplasmic reticulum , mutant , placenta , allantois , embryo , genetics , fetus , gene , pregnancy
The t rans m embrane e mp24 d omain/p24 (TMED) family are essential components of the vesicular transport machinery. TMED family members serve as cargo receptors important for selection and packaging of endoplasmic reticulum (ER) luminal proteins into coatomer (COP) II coated vesicles for anterograde transport to the Golgi. Until recently the role of TMED proteins in development and disease remained a mystery. We postulated that members of the TMED family regulate transport of cargo proteins essential for normal development and homeostasis. Tmed2 , the sole member of the vertebrate Tmed β‐subfamily, shows tissue and temporal specific patterns of expression during embryogenesis, but is ubiquitously expressed in all adult organs. We previously showed that mouse embryos homozygous mutant for a single point mutation, the 99J mutation, in the signal sequence of Tmed2 fail to form a functional placenta, and that heterozygous mice carrying this mutation developed non‐alcoholic liver disease. Using explants of chorion and allantois, the precursors of the developing placenta, we identified several secreted and membrane‐bound proteins that were abnormally expressed in Tmed2 mutant tissues, indicating a role for TMED2 in transport of secreted and membrane bound cargoes. In addition, explants between wild type chorion and Tmed2 homozygous mutant allantois, and vice‐versa, revealed both cell‐non‐autonomous and cell‐autonomous requirements for TMED2 during placental development. Our studies indicate that TMED2 is required for organogenesis of the placenta, which, is essential for survival of the growing fetus. Support or Funding Information Natural Sciences and Engineering Research Council This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .