Premium
Exercise characteristics in mitochondrial catalase overexpressing mice
Author(s) -
Rao Shriya D.,
Gaillard Charleston E.,
McKinley Alexander J.,
Klatt Benjamin T.,
Shanely R. Andrew,
Christian Brooke E.,
Zwetsloot Kevin A.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.771.7
Subject(s) - catalase , skeletal muscle , medicine , vo2 max , treadmill , context (archaeology) , forelimb , endocrinology , endurance training , grip strength , chemistry , reactive oxygen species , oxidative stress , physical therapy , biochemistry , biology , heart rate , anatomy , blood pressure , paleontology
High intensity or prolonged exercise can result in oxidative stress due to the generation of free radicals and reactive oxygen species, which in turn, can negatively impact skeletal muscle contractile function and exercise performance. Catalase, a naturally‐occurring antioxidant enzyme, converts hydrogen peroxide to water and oxygen (2H 2 O 2 → 2H 2 O + O 2 ). Mitochondrial‐targeted catalase transgenic (mCAT) mice have been studied within the context of disease pathology, but skeletal muscle function and exercise capacity have not been characterized. Therefore, the purpose of this study was to measure grip strength, exercise capacity, and exercise endurance in mCAT mice that overexpress catalase in the mitochondria of skeletal muscle and heart, among other tissues. Male and female mCAT and wild‐type (WT) littermate mice (3–8 mo old) performed 3 consecutive forelimb grip strength tests, with 1‐min rest between trials. Next, these mice performed a maximal exercise capacity test, which consisted of a 10‐min warm up at 10–12.5 m•min −1 and 5–10° incline, followed by 2‐min stages of increasing treadmill speed by 3 m•min −1 every stage; treadmill remained at 10° incline and mice ran to exhaustion. Then, 48 hours after the completion of the maximal exercise capacity test, all mice performed an exercise endurance test, consisting of a 10‐min warm up at 10–12.5 m•min −1 and 5–10° incline, followed by a run to exhaustion at a constant treadmill speed of 22 m•min −1 and incline of 10°. Grip strength, maximal exercise capacity, and exercise endurance were not different between mCAT and WT mice, respectively (p>0.05, all). These findings suggest that mitochondrial catalase overexpression does not alter grip strength, treadmill exercise capacity, or endurance in mice. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .