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Effects of Creatine Supplementation and Doxorubicin Treatment on Myogenic Regulatory Factor Expression in Slow and Fast Muscle
Author(s) -
Torok Zoltan,
Busekrus Raquel,
Hydock David Scott
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.769.1
Subject(s) - myod , myogenin , myf5 , endocrinology , medicine , creatine , myogenic regulatory factors , intraperitoneal injection , hyperhomocysteinemia , chemistry , skeletal muscle , myogenesis , homocysteine
The purpose of this study was to investigate the effects of creatine monohydrate supplementation on myogenic regulatory factor expression in skeletal muscle from rats receiving the chemotherapy drug doxorubicin. Male Sprague‐Dawley rats were randomly assigned to the control (CON), doxorubicin (DOX), or creatine+doxorubicin (CR+DOX) groups. CR+DOX received rodent chow supplemented with 3% creatine monohydrate and the CON and DOX received standard rodent chow. After two weeks of feeding, CR+DOX and DOX groups received a bolus, 15 mg/kg intraperitoneal (i.p.) DOX injection and CON received an i.p. saline injection as a placebo. Dietary interventions then continued for five more days. The soleus and extensor digitorum longus (EDL) were excised five days post injection and expression of myogenic regulatory factors MyoD, Myf5, Mrf4, and myogenin was determined using Western blotting. In DOX solei, MyoD, Myf5, Mrf4, and myogenin expression was observed to be +25%, −10%, +5%, and −25%, respectively when compared to CON, and −6%, +11%, −17%, and −12%, respectively in CR+DOX solei when compared to CON. MyoD, Myf5, Mrf4, and myogenin expression in DOX EDL were observed to be −4%, −44%, −14%, and −29%, respectively when compared to CON and −24%, −58%, −11%, and −43%, respectively in the EDL from CR+DOX when compared to CON. In the soleus, a trend toward increased MyoD and Mrf4 expression and decreased Myf5 and myogenin expression was observed with DOX treatment. The trend of CR+DOX expressing increased soleus Myf5 expression when compared to both CON and DOX is of interest and should be explored further. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .