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Channel catfish GLUT4 mRNA expression in various tissues and muscle changes in response to changes in feeding frequency
Author(s) -
Schmidtberger Abigail Marie,
Dougherty Megan,
Abernathy Oaklee,
Nevarez Ericka,
Kostner Danica,
Spainhour Rebekah,
Kobayashi Yass
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.768.2
Subject(s) - glut4 , catfish , biology , glucose transporter , medicine , endocrinology , messenger rna , muscle tissue , insulin , biochemistry , gene , fish <actinopterygii> , fishery
Glucose transporter proteins (GLUT) facilitate the movement of glucose across cell membranes. Various GLUT proteins, including GLUT1, 2, and 4, are distributed in all tissues throughout the body. The activity and expression of GLUT4 is regulated by insulin, making it important in maintaining proper blood glucose levels in the body. Although the role of GLUT4 in metabolism has been well‐characterized in mammals, tissue distribution and relative changes in the mRNA expression of GLUT4 in channel catfish has not yet been examined. For this study, our objectives were to characterize GLUT4 in channel catfish by examining its expression in various tissues. Tissue distribution of GLUT4 mRNA was examined in the brain, heart, liver, muscle, spleen, kidney, small intestine, and large intestine by using quantitative RT‐PCR. The expression of GLUT4 mRNA was detectable in all of the tissues examined. However, its expression was highly detectable in the brain, muscle, spleen, kidney, and large intestine compared to the other tissues. Changes in muscle GLUT4 mRNA expression in response to changes in feeding frequency were also examined. Juvenile catfish were assigned to receive food once every 12, 24, or 48 hours for 28 days (n=4 tanks per treatment, 8 fish per tank). Expression of GLUT4 mRNA was measured from muscle samples after the completion of the study (n=2 fish per tank). Muscle GLUT4 mRNA was not affected by changes in feeding frequency (P>0.10). We are currently examining the effects of feeding frequency on GLUT4 mRNA expression in the brain of channel catfish. Support or Funding Information This project was supported by the Institutional Development Award (IDeA) from the National Institute of General Medical Sciences of the National Institutes of Health under grant number P20GM103418. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .