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Ectonucleotidases in the Hypothalamus of Salt‐Induced Hypertensive Rats: Transcriptional Profile and functional implications
Author(s) -
Sá Renato Willian Martins,
Ribeiro Natália,
Ribeiro Luciene Maria,
Mendonça Johnny Mendes,
Santos Karol Martins,
Antunes Vagner Roberto
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.732.9
Subject(s) - extracellular , medicine , endocrinology , hypothalamus , tonicity , receptor , chemistry , blood pressure , adenosine , enzyme , biochemistry
Ectonucleotidases consist of families of nucleotide metabolizing enzymes that hydrolyze extracellular nucleotides, such as ATP, to nucleosides. At the CNS level, these enzymes can affect the excitability of hypothalamic neurons by controlling the extracellular availability of ATP and adenosine. ATP, acting on P2 receptor in the PVN neurons, is involved in hyperosmolality‐induced sympathoexcitation and hypertension in rats. Therefore, we hypothesized that elevated sympathetic activity in salt‐induced hypertensive rats occurs in parallel to a suppression of ectonucleotidases gene expression at the PVN that failure to breakdown ATP. To test this hypothesis, we have used 2 cohorts of male Wistar rats: control (C; tap water; n=5) and salt‐loaded with hypertonic solution (HS; NaCl 2%; n=7) in replacement of the tap water for 7 days. The arterial blood pressure (ABP) was monitored 24‐hour by day during the 7 days via telemetry system. Low frequency (LF) component of systolic ABP (SBP) variability was measured by spectral analysis. PVN mRNA levels for E‐NTPDase 1–3 and TMPAP were quantified using real time qPCR. All experimental procedures were approved by the ethical committee for animal research of the ICB‐USP (n° 38/2016). After 7 days of high‐salt intake the ABP of the HS group was higher (129±3 mmHg, p<0.05) compared to the C group (104±3 mmHg). The LF of SBP variability was higher in HS compared to C group [(1.33±0.23 mmHg 2 (C) and 4.36±1.22 mmHg 2 (LF)]. mRNA level for E‐NTPDase 3 was higher in HS than in C group (3,09±0,6 ΔΔCt vs 0,99±0,5 ΔΔCt, respectively), and TMPAP expression was very low in HS group. mRNA levels of the E‐NTPDase 1,2 did not differ between the groups. The data indicate that high expression of E‐NTPDase3 mRNA may be correlated to a greater ATP breakdown at PVN level of salt‐induced hypertensive rats. Support or Funding Information Financial support: FAPESP #2016/21991‐3 #2016/03359‐8; CAPES This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .