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Elevated PBMC‐derived oxidative stress in healthy young African American women
Author(s) -
Johnson Auburn L.,
Vranish Jennifer R.,
Kaur Jasdeep,
Liu Xian,
Blankenship Jeanette K.,
Pan Zui,
Fadel Paul J.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.730.7
Subject(s) - superoxide , peripheral blood mononuclear cell , oxidative stress , endocrinology , medicine , blood pressure , reactive oxygen species , intracellular , immunology , superoxide dismutase , chemistry , physiology , biochemistry , enzyme , in vitro
Several studies have demonstrated that African Americans (AA) exhibit elevations in systemic oxidative stress compared to Caucasian Americans (CA). Peripheral blood mononuclear cells (PBMCs) have been identified as one of the primary contributors to systemic reactive oxygen species (i.e. oxidative stress) and may be an underlying mechanism for the development of hypertension and cardiovascular diseases. Previous work demonstrates that young healthy AA men have elevated PBMC‐derived superoxide production when compared to CA men. However, whether PBMC‐derived superoxide production is also elevated in young healthy AA women remains unknown. This is important because AA women are also more likely to develop hypertension and cardiovascular diseases than women of any other race. Accordingly, this study investigated PBMC‐derived superoxide production in young healthy AA and CA women. We tested the hypothesis that, relative to CA women, AA women would exhibit greater levels of PBMC‐derived oxidative stress. In ten normotensive AA women and ten age‐matched normotensive CA women, resting intracellular superoxide levels were assessed from freshly isolated PBMCs using dihydroethidium fluorescence. Subjects arrived to the lab following an overnight fast. Resting blood pressure was measured following a 10‐minute rest period and a 50‐mL venous blood sample was then drawn. PBMCs were isolated from whole blood using density gradient centrifugation and intracellular superoxide was assessed using dihydroethidium fluorescence. Resting mean arterial pressure was similar between groups (AA 82.9±1.7 vs. CA 79.4±2.4 mmHg; P =0.26) however the resting intracellular superoxide production was elevated in AA women compared to CA (AA 4.1±1.9 vs. CA 2.7±1.0 Relative Fluorescent Units; P =0.025). These preliminary findings suggest that young AA women exhibit greater resting PBMC‐derived superoxide production. Thus, PBMCs may represent a source of elevated oxidative stress in AA women. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .