Premium
Role of reactive oxygen species in gestational diabetes and the impact on the developing fetus
Author(s) -
Arriaga Diana
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.719.17
Subject(s) - gestational diabetes , endocrinology , offspring , medicine , fetus , reactive oxygen species , diabetes mellitus , insulin , biology , placenta , hormone , gestation , pregnancy , biochemistry , genetics
Gestational diabetes mellites (GDM) is characterized by elevated levels of glucose in a pregnant woman's bloodstream that occurs from an overproduction of hormones. Under GDM, elevated glucocorticoids (GC) and insulin like growth factor (IGF)‐1 hormones in the placenta may possibly lead to an increase in the amount of reactive oxygen species (ROS) in the embryo due to an increase in glucose utilization and mitochondrial activity. Overproduction of ROS can modify the genome, thus predisposing offspring's to diabetes and obesity later. The aim of the study is to investigate the mechanism by which GDM leads to an increased production of ROS and evaluate the epigenetic changes that occur. I hypothesize that under diabetic conditions, the embryo will have an increase in ROS production due to increased hormone levels, resulting in epigenetic changes in the offspring. An in vivo model for GDM will be established by feeding female mice a high‐fat diet for four weeks. Controls will be fed a standard diet. Fasting blood glucose levels and glucose tolerance tests will monitor the development of diabetes. Mating between a diabetic or control female will occur with a normal male. At 6.5 and 17.5 days of gestation and 7 days post birth, maternal, fetal and placental blood and tissue will be collected. Biochemical assays will evaluate tissue ROS levels and epigenetic changes. Blood analysis will measure hormone and glucose levels. Results from this study will hopefully lead to the development of treatments to lower the incidence of diabetes in future generations. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .