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AMP‐activated protein kinase activation by traditional herbal medicines and their active compounds ameliorates oxidative stress as mediated with liver kinase B1 and farnesoid X receptor
Author(s) -
Baek Su Youn,
Lee Eun Hye,
Kim Young Woo
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.702.6
Subject(s) - oxidative stress , arachidonic acid , pharmacology , liver injury , reactive oxygen species , mitochondrial permeability transition pore , chemistry , protein kinase a , glutathione , biochemistry , kinase , medicine , apoptosis , programmed cell death , enzyme
Background Oxidative stress is a disorder in the oxidant‐antioxidant balance potentially leading to cellular damage in human. In the liver, oxidative stress stimulated by iron accumulation can induce a variety of chronic liver diseases, including liver fibrosis, cirrhosis and cancer. Oxidative stress resulting from iron accumulation may induce the release of arachidonic acid, and excessive amounts of arachidonic acid can cause high levels of cellular and mitochondrial ROS that reduce the functioning of mitochondrial respiration in the liver. Methods This study was designed to examine the effects of traditional herbal medicines and their active compounds against severe oxidative stress and mitochondrial impairment and its cellular mechanisms of action in vivo and in vitro . Results In cell experiments, the medicinal herbs such as Fructus Ligustri lucidi and Glycyrrihiza Radix effectively protected hepatocyte from arachidonic acid + iron‐induced oxidative stress and apoptosis, as indicated by depletion of glutathione, formation of reactive oxygen species, increases in mitochondrial membrane permeability and altered expression of apoptosis‐related proteins, all of which are similar with aminoimidazole‐carboxamide ribonucletide treatment, as a positive control. In mice, The administration of the drug candidates also protected against oxidative stress and liver injury induced by carbon tetrachloride injection, as indicated by decreases in histological liver damage and serum alanine amino transferase level. Moreover, active compounds from the herbs also induced the activation of AMP‐activated protein kinase, which was mediated by its upstream kinase liver kinase B1 and farnesoid X receptor .Conclusions Our results confirmed that beneficial herbs have the ability to protect hepatocytes and liver against oxidative injury through regulation of the AMP‐activated protein kinase signaling pathway. This work was supported by the National Research Foundation of Korea grant funded by the Korea government [MSIP] (No. 2017R1D1A3B03027847) and (2015K1A3A1A59069800). All authors disclose no conflict of interests. Support or Funding Information This work was supported by the National Research Foundation of Korea grant funded by the Korea government [MSIP] (No. 2017R1D1A3B03027847) and (2015K1A3A1A59069800). All authors disclose no conflict of interests. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .