Premium
Inhibitory effect and mechanisms of Crossostephium chinensis (L.) Makino on cell proliferation and migration of vascular smooth muscle cells
Author(s) -
Wang Jieyu,
Wu ChiehHsi
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.700.3
Subject(s) - restenosis , viability assay , vascular smooth muscle , medicine , intimal hyperplasia , traditional medicine , protein kinase b , coronary arteries , pi3k/akt/mtor pathway , pharmacology , western blot , inhibitory postsynaptic potential , smooth muscle , cell , chemistry , signal transduction , artery , biochemistry , stent , gene
Percutaneous transluminal coronary angioplasty (PTCA) is a common angioplasty used to relieve the narrowing and obstruction of coronary arteries. Restenosis occurs 30% to 50% of patients after PTCA. The mechanism of restenosis is principally neointinal hyperplasia and inflammatory, as stents resist arterial remodeling. Crossostephium chinensis , a traditional herbal medicine, is indicated on arthralgia, dehumidify and cure rheumatism. Previous studies showed that the extracts of C. chinensis have antioxidant, anti‐proliferative and anti‐inflammatory activities. Thereby, the purpose of this study was to explore the potential effect and mechanisms of C. chinensis on restenosis. Cell viability and migration were examined by MTT and trans‐well assays, respectively. The protein level was monitored by Western blot. Experimental data showed that extracts of C. chinensis can suppress the vascular smooth muscle cells (VSMCs) viability and migration. Protein levels of phospho‐AKT, mTOR, FAK, ERK, PDGFR‐β had been reduced by extract of C. chinensis . Our study suggested that C. chinensis can be a potential inhibitory remedy for restenosis. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .