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Syndecan‐4 and PECAM‐1‐Dependent Cell Motility
Author(s) -
Abraham Valsamma,
Parambath Andrew,
Murarka Monisha,
Zoga Kristi,
Saeed Danyall,
Tran Thuongvu,
Kumar Rahul,
DeLisser Horace
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.676.12
Subject(s) - syndecan 1 , cdc42 , microbiology and biotechnology , heparan sulfate , motility , chemistry , angiogenesis , cell migration , filopodia , cell , biology , biochemistry , signal transduction , cancer research
Ligand interactions between PECAM‐1 and heparan sulfate proteoglycans have been implicated in the ability of PECAM‐1 to promote cell motility by stimulating Cdc42 activation and the extension of filopodia. However, the endothelial proteoglycan(s), involved in these activities are undefined. We therefore investigated the potential involvement of syndecans (SDCs), a family of four cell surface heparan sulfate proteoglycans, in these PECAM‐1‐dependent processes. For these studies, we employed HUVEC and REN cells (an EC surrogate) expressing mouse PECAM‐1 (REN‐MP). For both cell types, we observed that while syndecan‐1 (SDC1) and syndecan‐4 (SDC4) are surface expressed, SDC4, but not SDC1, co‐immunoprecipitated with PECAM‐1. This PECAM‐1/SDC4 interaction was disrupted by targeting PECAM‐1‐dependent, heparan/glycosaminoglycan (GAG) ligand binding. Further, antibody‐ and/or siRNA‐mediated inhibition of SDC4 in REN‐MP cells inhibited PECAM‐1‐dependent, heparan/GAG heterophilic binding, cell motility, filopodia extension and Cdc42 activation. The targeting of SDC4 did not suppress the concentration of PECAM‐1 in the tips of filopodia, a process previously shown to be mediated by PECAM‐1‐PECAM‐1 homophilic, but not heterophilic, ligand binding. These data suggest that cis binding interactions between PECAM‐1 and SDC4 in the membrane of endothelial cells are involved in PECAM‐1‐dependent cell motility during angiogenesis. Support or Funding Information Veterans Administration Merit Award This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .