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Clinical Biomarkers of Congestive Heart Failure Significantly Correlate with Pancreatic Trypsin and Lipase Levels in Human Blood Plasma
Author(s) -
Courelli Vasiliki,
Courelli Asimina,
Mills Paul,
SchmidSchoenbein Geert
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.675.6
Subject(s) - medicine , endocrinology , heart failure , trypsin , lipase , pancreatitis , pancreas , chemistry , enzyme , biochemistry
We recently have shown that pancreatic enzymes entering the circulation after a rise in intestinal permeability may cause multiple organ dysfunctions. Increased intestinal permeability occurs in congestive heart failure (CHF), for example due to reduction of cardiac output with hypoperfusion of the small intestine. We hypothesize that elevated intestinal permeability during CHF allows digestive enzymes, such as pancreatic trypsin and lipase, typically found in the lumen of the small intestine, to enter the systemic circulation. Our goal is to determine the levels of pancreatic trypsin and lipase and their correlation with clinical biomarkers of inflammation, and functional measurements of the heart and sympathetic nervous system. Blood from 35 (19 CHF and 16 non‐CHF Controls) patients at rest and analyzed by Western Blot for pancreatic lipase and trypsin and for inflammatory markers by ELISA assays for TNF‐a, IL‐6, BNP, ICAM, p‐Selectin, CRP, differential leukocyte counts and adrenergic sensitivity assays using isoproterenol. Clinical measurements such as age, body weight, BMI, and cardiac ejection fraction were recorded. The results showed significantly higher levels of trypsin, lipase, as well as TNF‐a, IL‐6, BNP, p‐Selectin, CRP, and neutrophil count. No change in monocyte, eosinophil, and basophil counts of CHF patients were seen when compared to Control. There were significant correlations between pancreatic trypsin levels and TNF‐a, BNP, and neutrophil count, and also between pancreatic lipase and TNF‐a, IL‐6, BNP, CRP, p‐Selectin, ejection fraction, neutrophil count, and Isoproterenol response. These results suggest that the protein levels of pancreatic digestive enzymes in the systemic circulation are elevated. The levels correlate with functional metrics of CHF and potentially play key roles as CHF biomarkers and in the analysis of the loss of cardiac function. Support or Funding Information National Institutes of Health Grant GM 85072 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .