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Protective Effect of Curcumin against Nitrosamine‐induced Gastric Cancer in Rats
Author(s) -
Waly Mostafa I.,
AlHinai Shaimaa,
AlBulushi Ismail,
Rahman Mohammad Shafiur
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.674.13
Subject(s) - nitrosamine , curcumin , carcinogen , oxidative stress , cancer , antioxidant , glutathione , carcinogenesis , pharmacology , chemistry , stomach , medicine , biochemistry , enzyme
Background Nitrosamine acts as a carcinogenic agent and induces gastric cancer in rats. The mechanisms underlies nitrosamine‐mediated gastric cancer pathogenesis need to be investigated. Experimental studies continue to support the notion that curcumin combat cancer by acting as a dietary antioxidant. Objective Our study model was undertaken to investigate the preventive effect of curcumin supplementation against nitrosamine‐induced oxidative stress and carcinogenesis in rat stomach. Methods Forty Sprague‐Dawley rats (8 weeks old) were randomly divided into 4 groups (10 rats/group). Control group was fed a standard rat chow diet; nitrosamine‐treated group was fed a standard rat chow diet and also received intraperitonial injections of nitrosamine. The other two groups received oral supplementations of curcumin in the presence or absence of nitrosamine injection. All animals were fed ad‐libitum for 16 weeks. At the end of the experiment all rats were sacrificed and the gastric tissues were examined microscopically for gastric cancer lesions development, and for biochemical measurements of oxidative stress indices. Results Our results showed that nitrosamine causes gastric cancer development, as well as inducing oxidative stress in gastric tissue as evidenced by glutathione depletion and inhibition of antioxidant enzymes. The concomitant treatment of nitrosamine with curcumin significantly ameliorated the cytotoxic effects of nitrosamine. Conclusion The results of this study provide in vivo evidence that curcumin supplementation protects against nitrosamine‐induced gastric cancer in the used animal model. Support or Funding Information This research was supported by Strategic Grant Funding (CL/SQU‐UAEU/15/03) and Internal Grant Funding (IG/AGR/FOOD/17/1), College of Agricultural and Marine Sciences, Sultan Qaboos University This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .