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Combining mass spectrometry and glycan array data to explore invertebrate glycomes
Author(s) -
Wilson Iain,
Eckmair Barbara,
Hykollari Alba,
Yan Shi,
Vanbeselaere Jorick,
Paschinger Katharina
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.673.23
Subject(s) - glycan , biology , computational biology , invertebrate , biochemistry , ecology , glycoprotein
As our glycoanalytical possibilities increase and the amounts of glycomic data on non‐mammalian species accumulate, we can start to consider whether the structures of glycoconjugates from invertebrates can be fitted into a sort of glycophylogeny and which protein receptors recognise them. Our recent data show that N‐glycans from a variety of nematode, mollusc and insect species contain a range of core and antennal modifications with fucose, galactose, glucuronic acid, sulphate, phosphoethanolamine, aminoethylphosphonate and phosphorylcholine. On one hand, we employ an off‐line HPLC/MALDI‐TOF‐MS workflow to assess the actual glycan structures; on the other, beyond previous Western blotting data, we are now printing invertebrate N‐glycomes in a microarray format. Thereby, we are uncovering interactions with different lectins, pentraxins and antisera. This approach should allow us to explore whether and how invertebrate glycomes, especially from medically or biotechnologically relevant species, interact with mammalian immune systems. Support or Funding Information This work is funded by the Austrian Science Fund (FWF) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .