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Harnessing glycocalyx interactions to modulate differentiation and development
Author(s) -
Huang Mia L.,
Michalak Austen L.,
Tota Ember M.,
Smith Raymond Alexander,
Trieger Greg W.,
Godula Kamil
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.673.16
Subject(s) - embryonic stem cell , microbiology and biotechnology , stem cell , biology , function (biology) , cellular differentiation , glycocalyx , cell fate determination , glycosaminoglycan , cell , computational biology , genetics , biochemistry , gene , transcription factor
The cellular fate of embryonic stem cells and their subsequent development into differentiated cells are governed by precise growth factor signaling events at the stem cell surface. Such events are often orchestrated by heparan sulfate proteoglycans (HSPGs), which template growth factor binding to their corresponding receptors. Given the structural complexity of HSPGs, however, methods to manipulate them to study their function has been limited. Here, we report a suite of chemical strategies that exploit the function of HSPGs to control pluripotency or trigger the differentiation of mouse embryonic stem cells. To this end, we employ small molecules targeting the glycosaminoglycan (GAG) side chains of HSPGs to maintain pluripotency, and design GAG‐mimetic molecules that can recapitulate the functions of native GAGs. Support or Funding Information NIH Pathway to Independence Award (1K99HD09029201, to MLH) NIH Director's New Innovator Award (1DP2HD08795401, to KG) This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .