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Designing a Model Skin Cell Membrane to Investigate the Extent of Nanoparticle Absorption
Author(s) -
Yoder Brandi,
Sostarecz Audra Goach
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.671.8
Subject(s) - stratum corneum , liposome , ceramide , membrane , biophysics , lipid bilayer , chemistry , membrane fluidity , monolayer , cell membrane , absorption (acoustics) , nanoparticle , fluorescence microscope , fluorescence , biochemistry , nanotechnology , materials science , biology , apoptosis , genetics , physics , quantum mechanics , composite material
The Langmuir Monolayer Technique is a useful method for modeling the epithelial cell membrane and for investigating the organization of membrane molecules. Liposomes with fluorescence analysis are useful in interpreting molecule interactions with the hydrophilic and hydrophobic regions of the membrane. Zinc oxide particles were introduced to monolayers of lipids found in the stratum corneum and also to liposomes of the same composition to investigate how these particles interact within the cell membrane. This process will allow us to ultimately determine if the nanoparticles in over the counter beauty products embed our skin. In our lab, the compression isotherms of four lipids, cholesterol, cholesterol sulfate, lignoceric acid, and N‐oleoyl‐d‐sphingosine, which are major components of the epithelial cell membrane have been established at body temperature conditions. Ceramide molecules have also been analyzed and incorporated to create a more realistic model system. Liposomes were used as a technique to study the bilayer due to the ability to analyze them via fluorescence spectroscopy by incorporation of a fluorescent ceramide. The ultimate goal is to determine whether or not the addition of the zinc oxide will increase the fluidity of our membrane system utilized for modeling epithelial cells. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .