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Mechanism of Membrane Biogenesis
Author(s) -
Naik Akshata Ramesh,
Kuhn Eric R.,
Lewis Kenneth T.,
Kokotovich Keith M.,
Maddipati Krishna R.,
Chen Xuequn,
Hörber Heinrich,
Taatjes Douglas J.,
Potoff Jeffrey J.,
Jena Bhanu P.
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.671.11
Subject(s) - membrane , membrane biology , intracellular , vesicle , microbiology and biotechnology , chemistry , biological membrane , lipid bilayer fusion , cell membrane , biology , biochemistry
Biological membrane in addition to delimiting the cell and its intracellular compartments, govern a wide range of life processes including ion transport, cell division, development, growth, and migration. The addition of new membrane to existing cellular membranes may involve fusion of preformed proteoliposomes and or the incorporation of intracellular free lipids and proteins. It is reported that supported lipid bilayers with adhered lipid vesicles when laterally stretched, expand due to fusion of the adhered vesicles. Mathematical modeling of the plasma membrane suggests trafficking of lipids into stretched membranes to relieve tension. Cell swelling has been reported during mitosis and mechanical forces are implicated in morphogenesis and development. However, the process of addition of new membrane to preexisting membranes in live cells is poorly understood. Here we report in live cells, that in addition to membrane stretch, composition of the existing membrane dictates membrane growth through the selective incorporation of both proteins and lipids. Support or Funding Information Work presented in this article was supported in part by the National Science Foundation grants EB00303, CBET1066661 (BPJ); the WSU Interdisciplinary Biomedical Systems Fellow‐ship and Graduate Research Assistantship (ARN), the WSU Interdisciplinary Biomedical Systems Fellow‐ship and Graduate Research Assistantship (KTL). All authors critically analyzed results and proof read the manuscript. The authors declare no competing financial interests or conflicts. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .