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Does this gene make me look fat? ATP10A Expression in Targeted Human Populations
Author(s) -
Hurst Sarah Elizabeth,
Ward Taketa,
Hall Brandon,
Smolinski Kasia
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.670.59
Subject(s) - overweight , obesity , body mass index , medicine , waist , type 2 diabetes , population , saliva , diabetes mellitus , endocrinology , physiology , biology , environmental health
Globally, the prevalence of type 2 diabetes (T2D) and diet‐induced obesity (DIO) is increasing due to higher energy intake and lower energy expenditure. However, the mechanisms that underlie these diseases remain unclear as numerous genes are implicated. ATP10A has been associated with several metabolic abnormalities in animal and in vitro models, so for this pilot study, we sought to identify the expression of ATP10A in targeted human populations. Based on the responses from the medical surveys, subjects were divided into three target groups based on body mass index (BMI), and five groups based on waist‐to‐height ratio (WtHR). From collected saliva samples, total RNA were extracted and analyzed using semi quantitative real‐time PCR (qRT‐PCR). Our results showed significant differences in the BMI groups by one‐way ANOVA [F (2, 6) = 49853, p < 0.0001] with the highest fold change in ATP10A seen in the overweight population (58.12) and the lowest in the normal weight population (32.28). When classified using WtHR, results again showed significance [F (4, 10) = 944735, p < 0.0001] with the overweight, obese, and highly obese groups having greater fold changes (10.56, 9.15, and 11.96 respectively) when compared to slender (0.19) and healthy groups (0.77). Collectively, these results suggest that ATP10A may play an important role in obesity and other metabolic disorders, and as such, we believe the results from this pilot study are promising and worth further exploration. Support or Funding Information Funding for this work was provided by donations to The ATP10A project. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .