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The role of ANKDR49 in evaluating the prognosis of glioma
Author(s) -
Duan Hubin,
Hao Chunyan,
Wang suping,
Wang Huan,
Gao Ling,
Zheng Xiaojun,
Duan Shengqiang,
Gao Weiqin,
Zhang Ce
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.663.2
Subject(s) - glioma , western blot , gene expression , gene , biology , cancer research , genetics
Objective To evaluate the role of ANKRD49 in the prognosis of glioma. Methods Transcriptome expression data sets and the corresponding clinical information of ANKRD49 gene were downloaded from the website of The Cancer Genome Atlas (TCGA). A total of 558 samples, which contained transcriptional expression data of 548 tumor tissues and 10 normal tissues, were available for this analysis. Human malignant glioma cell lines U251 and U87 were cultured. Total RNA was isolated from indicated cells using TRIzol reagent. Quantitative real‐time PCR was used to analyze ANKRD49 gene expression. Total protein was isolated from indicated cells and West‐blot was used to analyze ANKRD49 protein expression. SPSS 16.0 software was used to analyze experimental data and clinical data. Results The expression of ANKRD49 was predominantly higher in glioma samples compared with matched normal samples(fold change =2.11, P=2.99E‐09). The 5‐year overall survival of patients with highly expressed ANKDR49 was distinctively shorter than that of patients with lowly expressed ANKDR49(P<0.01). Conclusion ANKRD49 is overexpressed in human glioma tissues, and high expression of ANKRD49 is significantly associated with poor survival. ANKDR49 might be a indicator for evaluating the prognosis of glioma. Support or Funding Information This study was supported by the National Natural Science Foundation Youth Fund (Fund No. 30600637), China Postdoctral Foundation(Fund No.2014M561207), the Shanxi Province Basic Research Program Youth Science and Technology Research Fund (Fund No. 2010021034‐4), the Shanxi Scholarship Council of China (Fund No. 2011‐096 and Key Projects No.4), The science and technology research projects of health department of Shanxi Province(Fund No.2016D011119), The Teaching Reform Program of Shanxi Higher Education (No. 2013‐033), The Education Reform Project of Postgraduate Students in Shanxi Province (No. 2017‐027). The Project of “131” Leading Talents of Shanxi Higher Education, The Education Reform Project of Shanxi Medical University. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .