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DNA binding properties of the transcription factor optix
Author(s) -
Rodriguez Jessica Marie
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.652.41
Subject(s) - biology , transcription factor , dna , dna binding site , genetics , computational biology , systematic evolution of ligands by exponential enrichment , gene , evolutionary biology , gene expression , promoter , rna
DNA binding proteins such as transcription factors are key determinants of cellular state and have been shown to control cell differentiation. Evaluating the specificity profile of DNA binding proteins is a nontrivial challenge that hinders the ability to decipher gene regulatory networks or engineer molecules that act on genomes. optix is a transcription factors that has been previously identified as a master regulator of red color patterns in the butterfly (Heliconius) developing wing. However the direct gene targets of optix and their effects in final wing coloration remain to be determined. Based on this, we want to determine the intrinsic DNA‐binding preferences of optix. We are currently working on cloning, over‐expressing and purifying full‐length optix and optix's DNA‐binding domain, the homeodomain (HD). We will determine the in vitro DNA‐binding specificity of optix protein of Heliconius erato using Cognate Site Identification by high‐throughput SELEX. Our results will enable us to bioinformatically predict the genomic targets of optix and to better understand its role in wing color patterning. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .