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Pervasive, coordinated protein level changes driven by transcript isoform switching
Author(s) -
Brar Gloria,
Otto George,
Cheng Ze,
Powers Emily,
Keskin Abdurrahman,
Jovanovic Marko
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.651.9
Subject(s) - gene isoform , gene , messenger rna , budding yeast , biology , transcription (linguistics) , genetics , saccharomyces cerevisiae , linguistics , philosophy
To better understand the gene regulatory mechanisms that program developmental processes, we carried out simultaneous, genome‐wide measurements of mRNA, translation and protein through meiotic differentiation in budding yeast. Surprisingly, we observed that the levels of several hundred mRNAs are anti‐correlated with their corresponding protein products. We show that rather than arising from canonical forms of gene regulatory control, the regulation of at least 380 such cases—or over 8% of all measured genes—involves temporally regulated switching between production of a canonical, translatable transcript and a 5′ extended isoform that is not efficiently translated into protein. By this pervasive mechanism for the modulation of protein levels through a natural developmental program, a single transcription factor can coordinately activate and repress protein synthesis for distinct sets of genes. The distinction is not based on whether an mRNA is induced or not, but rather based on the type of transcript produced. Support or Funding Information This work was supported by NIH funding to G.A.B. (DP2‐GM‐119138), investigator awards from the Alfred P. Sloan Foundation and Pew Charitable Trusts to G.A.B., UC‐Berkeley start‐up funding to G.A.B. G.M.O. is supported by an NIH training grant (GM0007232) to UCB MCB. E.P. is supported by an NSF predoctoral fellowship. M.J. was supported by the Marie Sklodowska‐Curie IOF and he and A.K. are supported by Columbia start‐up funding. This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .