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Systematic Screening for Transcriptional Regulators of Adult Myogenesis in Drosophila by RNAi
Author(s) -
Soudachanh Tommy,
Oas Sandy,
Mendes Tyler,
Byrantsev Anton,
Cripps Richard
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.648.13
Subject(s) - biology , myogenesis , enhancer , rna interference , phenotype , gene , myocyte , microbiology and biotechnology , genetics , transgene , drosophila melanogaster , transcription factor , rna
The muscles of Drosophila show similar structure and pathway of development to the muscles of humans, and allows Drosophila to model human muscle disorders. However, little is known about many of the genes and their potential role in muscle development. Thus, the focus of this project is to efficiently screen for potential transcriptional regulators of muscle development and identify associated disorder phenotypes. Genes were selected based on having a potential role in transcription, which were silenced using RNA interference (RNAi). The 1151‐gal4 driver was utilized to induce RNAi in developing adult muscles. Combined with the driver, the flies harbored enhancers of either Flightin‐LacZ or TpnC41C‐LacZ . The resulting transgenic flies were collected and assayed to measure the effects of this genetic loss. All 101 genes in the latest set have been assayed for a change of enhancer activity of less than 70% or greater than 130%, in Flightin and TpnC41C ; the genes selected for immunostaining exhibited semi‐lethality or lethality, and indicated a loss or gain of LacZ. The immunostaining would reveal several factors: muscle morphology, and expansion or loss of enhancer activity associated with specific flight and jump muscle genes. The resulting data was classified into three categories, severe loss, moderate loss, or no change in muscle development. Genes with a severe loss indicates a potential issue with myoblasts fusion, and genes with a moderate loss indicates issues with adult muscle formation and maturation. Therefore, the results demonstrate that the screen is efficient in identifying potential transcriptional regulators and muscle disorder phenotypes. Support or Funding Information National Institute of Health ROI GM061738 This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .