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Informative Three‐dimensional Survey of Cell/tissue Architectures in Thick Paraffin Sections by Simple Low‐vacuum Scanning Electron Microscopy
Author(s) -
Sawaguchi Akira,
Kamimura Takeshi,
Yamashita Atsushi,
Takahashi Nobuyasu,
Ichikawa Kaori,
Aoyama Fimiyo,
Asada Yujiro
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.642.5
Subject(s) - electron microscope , scanning electron microscope , biomedical engineering , microscopy , materials science , thrombus , pathology , chemistry , biophysics , optics , biology , medicine , physics , composite material , surgery
Recent advances in bio‐medical researches, such as the production of regenerative organ from stem cells, require three‐dimensional analysis in those cell/tissue architectures. High‐resolution imaging by electron microscopy remains superior to elucidate complex cell/tissue architectures, but the conventional method requires skillful and time‐consuming preparation. The present study developed a three‐dimensional survey of the cell/tissue architectures in 30 μm‐thick paraffin sections, by taking the advantages of backscattered electron imaging in low‐vacuum scanning electron microscope. As results, in the kidney, the podocytes and their processes were clearly observed covering the glomerulus. The 30 μm‐thickness facilitated investigation on a face‐side, instead of sectioned, image of the epithelium and endothelium that is rarely seen within conventional thin section. In the testis, differentiated sperms were three‐dimensionally assembled in the middle of seminiferous tubule. Further application to vascular injury thrombus formation revealed the distinctive networks of fibrin fibers and platelets, capturing the erythrocytes into the thrombus. The four segmented BSE detector provided topographic bird's‐eye images for three‐dimensional understanding of the cell/tissue architectures at electron microscopic level. Here we describe the precise procedures accompanied by representative electron micrographs of normal rat organs, experimental thrombus formation, and three‐dimensionally cultured tumor cells. Support or Funding Information Ministry of Education, Culture, Sports, Science and Technology(MEXT), JapanThree‐dimensional survey of cell/tissue architectures in thick paraffin sections by simple low‐vacuum scanning electron microscopyThis abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .