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Characterization of Urinary Exosomes from Diabetic Hypertensive db/db Mice
Author(s) -
Schramm Whitney,
Tuna Kubra,
Chacko Kevin,
Sanchez Victor,
Ahmed Hamzah,
Liu Lauren,
Alli Abdel
Publication year - 2018
Publication title -
the faseb journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.709
H-Index - 277
eISSN - 1530-6860
pISSN - 0892-6638
DOI - 10.1096/fasebj.2018.32.1_supplement.624.35
Subject(s) - microvesicles , western blot , diabetes mellitus , endocrinology , urinary system , medicine , type 2 diabetes , kidney , exosome , urine , blot , type 2 diabetes mellitus , chemistry , microrna , biochemistry , gene
Hypertension can occur before or after the onset of type 2 diabetes mellitus. The db/db mouse model is commonly used to study type 2 diabetes mellitus and its comorbidities including obesity and hypertension. Diabetic db/db mice develop salt‐sensitive hypertension after salt loading. Urinary exosomes are nano‐sized vesicles derived from cell types within the kidney that contain lipids, proteins, and nucleic acids. Recent studies showed urinary exosomes are useful biomarkers for kidney associated diseases including hypertension. We hypothesized the production of urinary exosomes is augmented and that their cargo is altered during the development of salt‐sensitive hypertension. Metabolic cage studies were performed to collect urine for the isolation of urinary exosomes from diabetic db/db mice and wild‐type littermate mice before and after salt‐loading. Western blot analysis was performed using validated antibodies to probe for the exosomal markers flotillin‐1, Hsp70, and annexin V in urinary exosomal lysates. NanoSight analysis showed an increase in the concentration of urinary exosomes from salt‐loaded, diabetic, hypertensive db/db mice and Western blot analysis revealed the enrichment of ENaC and MARCKS in these exosomes compared to non‐salt loaded diabetic db/db mice or healthy wild‐type littermate mice after salt‐loading. Future studies will investigate the physiological role of exosomal MARCKS in the development of diabetes associated hypertension. Support or Funding Information This research was supported by a National Institutes of Health grant K01 DK099617 (to A. A. Alli). This abstract is from the Experimental Biology 2018 Meeting. There is no full text article associated with this abstract published in The FASEB Journal .